normal human primary osteoblast hfob1 19 cells Search Results


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Genechem normal human osteoblast cell line hfob1.19
Normal Human Osteoblast Cell Line Hfob1.19, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nanjing KeyGen Biotech Co Ltd normal human osteoblast hfob1.19 cell
Normal Human Osteoblast Hfob1.19 Cell, supplied by Nanjing KeyGen Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Obio Technology Corp Ltd human osteoblast cell line hfob1.19
Human Osteoblast Cell Line Hfob1.19, supplied by Obio Technology Corp Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ScienCell human normal osteoblast hfob 1.19
Human Normal Osteoblast Hfob 1.19, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lonza human osteoblast cells line hfob 1.19
( A ) ALP activity showed an increase of calcification of the extracellular matrix after inclusion of GelMA. ( B ) Calcium deposition demonstrated a further influence of GelMA to enhance the functions of <t>osteoblasts.</t> ( C ) MTS assay showing that osteoblastic cells were further influenced by hydrophilic properties after inclusion of PEG and GelMA. Data plotted in mean and SD (N=5). Values of P <0.01 were considered significant. Data were normalized by the cells, and the y-axis was multiplied by 10 4 . For the ALP and calcium deposition, the data were compared to control (cells) and between each time. For cellular proliferation assays, the data were compared to pure PCL. N=5. ** P <0.01, *** P <0.001, and **** P <0.0001 mean statistical differences. SEM of hFOBs cultivated on scaffolds after 7 days. ( D ) (i) PCL and (ii) magnified view. ( E ) (i) PCL-PEG and (ii) magnified view. ( F ) (i) PCL-PEG-GelMA without UV crosslinking and (ii) magnified view. ( G ) (i) PCL-PEG-GelMA after UV crosslinking and (ii) magnified view. The cells are spreading on all produced scaffolds presenting filopodium and cytoplasmic extension. Abbreviations: ALP, alkaline phosphatase; GelMA, gelatin methacryloyl; hFOB, human osteoblasts; MTS, (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium); NS, no significance; PCL, polycaprolactone; PEG, poly(ethylene glycol); SEM, scanning electron microscopy.
Human Osteoblast Cells Line Hfob 1.19, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LGC Promochem human fetal osteoblast cell line hfob1.19
( A ) ALP activity showed an increase of calcification of the extracellular matrix after inclusion of GelMA. ( B ) Calcium deposition demonstrated a further influence of GelMA to enhance the functions of <t>osteoblasts.</t> ( C ) MTS assay showing that osteoblastic cells were further influenced by hydrophilic properties after inclusion of PEG and GelMA. Data plotted in mean and SD (N=5). Values of P <0.01 were considered significant. Data were normalized by the cells, and the y-axis was multiplied by 10 4 . For the ALP and calcium deposition, the data were compared to control (cells) and between each time. For cellular proliferation assays, the data were compared to pure PCL. N=5. ** P <0.01, *** P <0.001, and **** P <0.0001 mean statistical differences. SEM of hFOBs cultivated on scaffolds after 7 days. ( D ) (i) PCL and (ii) magnified view. ( E ) (i) PCL-PEG and (ii) magnified view. ( F ) (i) PCL-PEG-GelMA without UV crosslinking and (ii) magnified view. ( G ) (i) PCL-PEG-GelMA after UV crosslinking and (ii) magnified view. The cells are spreading on all produced scaffolds presenting filopodium and cytoplasmic extension. Abbreviations: ALP, alkaline phosphatase; GelMA, gelatin methacryloyl; hFOB, human osteoblasts; MTS, (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium); NS, no significance; PCL, polycaprolactone; PEG, poly(ethylene glycol); SEM, scanning electron microscopy.
Human Fetal Osteoblast Cell Line Hfob1.19, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) ALP activity showed an increase of calcification of the extracellular matrix after inclusion of GelMA. ( B ) Calcium deposition demonstrated a further influence of GelMA to enhance the functions of osteoblasts. ( C ) MTS assay showing that osteoblastic cells were further influenced by hydrophilic properties after inclusion of PEG and GelMA. Data plotted in mean and SD (N=5). Values of P <0.01 were considered significant. Data were normalized by the cells, and the y-axis was multiplied by 10 4 . For the ALP and calcium deposition, the data were compared to control (cells) and between each time. For cellular proliferation assays, the data were compared to pure PCL. N=5. ** P <0.01, *** P <0.001, and **** P <0.0001 mean statistical differences. SEM of hFOBs cultivated on scaffolds after 7 days. ( D ) (i) PCL and (ii) magnified view. ( E ) (i) PCL-PEG and (ii) magnified view. ( F ) (i) PCL-PEG-GelMA without UV crosslinking and (ii) magnified view. ( G ) (i) PCL-PEG-GelMA after UV crosslinking and (ii) magnified view. The cells are spreading on all produced scaffolds presenting filopodium and cytoplasmic extension. Abbreviations: ALP, alkaline phosphatase; GelMA, gelatin methacryloyl; hFOB, human osteoblasts; MTS, (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium); NS, no significance; PCL, polycaprolactone; PEG, poly(ethylene glycol); SEM, scanning electron microscopy.

Journal: International Journal of Nanomedicine

Article Title: Electrospun nanofiber blend with improved mechanical and biological performance

doi: 10.2147/IJN.S175619

Figure Lengend Snippet: ( A ) ALP activity showed an increase of calcification of the extracellular matrix after inclusion of GelMA. ( B ) Calcium deposition demonstrated a further influence of GelMA to enhance the functions of osteoblasts. ( C ) MTS assay showing that osteoblastic cells were further influenced by hydrophilic properties after inclusion of PEG and GelMA. Data plotted in mean and SD (N=5). Values of P <0.01 were considered significant. Data were normalized by the cells, and the y-axis was multiplied by 10 4 . For the ALP and calcium deposition, the data were compared to control (cells) and between each time. For cellular proliferation assays, the data were compared to pure PCL. N=5. ** P <0.01, *** P <0.001, and **** P <0.0001 mean statistical differences. SEM of hFOBs cultivated on scaffolds after 7 days. ( D ) (i) PCL and (ii) magnified view. ( E ) (i) PCL-PEG and (ii) magnified view. ( F ) (i) PCL-PEG-GelMA without UV crosslinking and (ii) magnified view. ( G ) (i) PCL-PEG-GelMA after UV crosslinking and (ii) magnified view. The cells are spreading on all produced scaffolds presenting filopodium and cytoplasmic extension. Abbreviations: ALP, alkaline phosphatase; GelMA, gelatin methacryloyl; hFOB, human osteoblasts; MTS, (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium); NS, no significance; PCL, polycaprolactone; PEG, poly(ethylene glycol); SEM, scanning electron microscopy.

Article Snippet: Totally 5,000 cells/cm 2 of human osteoblast cells line (hFOB 1.19, bone-forming cells; Lonza, CRL-11372, second passage) were cultured using C27015 media (Osteoblast Basal Medium, Promocell GmbH), 1% penicillin/streptomycin (P/S; Hyclone), and an Osteoblast Growth Medium Supplement Mix under standard cell culture conditions (37°C, 5% CO 2 , and 95% air).

Techniques: Activity Assay, MTS Assay, Control, Produced, Electron Microscopy